Lab unknown for bacillus subtilis jordan hansen unknown lab report professor zelman april 9, 2012 identifying an unknown bacteria introduction knowing the identity of a microorganism is extremely beneficial. Unknown b was determined to be gram + rods, which narrowed the candidates to b cereus and b subtilis this was confirmed with a positive simmon's citrate test a negative methyl red test identified unknown b as b subtilis. Unknown lab report unknown organism #6 ann le (phuoc) may 6, 2010 dr carrington microbiology lab- mw 12:50 le 1 i introduction my unknown organism #6 is morganella morganii, which is a gram-negative bacillus rods commonly found in the environment and also in the intestinal tracts of humans, mammals, and reptiles as a normal flora (3, 5) this. Macconkey agarbacillus subtilis was eliminated from the discussion because based on the experiment conducted by goldrick setlow (1983) bacillus subtilis has smaller size comparing to bacillus megaterium and the colonies shaped very differently through microscopes it can be concluded that bacteria 10 is bacillus megaterium. 2 introduction: bacillus subtilis (genus bacillus and species subtilis) is found in the kingdom bacteria, within the firmicutes phylum, in the bacilli class, in the bacillales order, and bacillaceae family.
Microbiology unknown lab report kateryna petrakova introduction microorganisms cause a great amount of diseases for healthcare providers it is very important to be aware of what organisms are pathogenic and cause a disease and, therefore, to find an appropriate treatment. Start studying micro lab final- all unknown bacteria learn vocabulary, terms, and more with flashcards, games, and other study tools. Unknown b- bacillus subtilis both unknown organisms provided by the instructor were identified using specific tests and interpreting the results of these tests unknown a, escherichia coli, was gram-stained to determine the bacterial shape.
Starch hydrolysis starch agar is a differential medium that tests the ability of an organism to produce certain exoenzymes, including a-amylase and oligo-1,6-glucosidase, that hydrolyze starch. Lab report of 7 possiable unknown bacteria (proteus vulgaris, staphylococcus epidermis, bacillus subtilis, escherichia coli, streptococcus agalactiae, moraxella catarrhalis, or enterobacter aerogenes. Bacillus cereus (b cereus) and bacillus subtilis (b subtilis) bacteria were left of the rod shaped bacterium's to be tested a milk agar plate was inoculated with the unknown isolation of bacterium because it differentiates by the production of the enzyme casease that hydrolyzes the milk protein casein. Choose and streak unknown #1 for isolation, label, write unknown number in notebook, incubate in 37c preserve original unknown #1 tube (labeled with name) in 4c fridge. Isolation and identification of gram (+) organism #13 - bacillus subtilis 4162012 - unknown organism was streaked from a mixed broth onto both tsa and bha plates in an attempt to isolate pure colonies for testing.
Learn morphology of unknown with free interactive flashcards choose from 37 different sets of morphology of unknown flashcards on quizlet. Bacillus cereus, bacillus coagulans, bacillus megaterium, bacillus subtilis, bacillus thuringiensis, brevibacillus brevis the test: the citrate test was done, this test determines if the specimen uses citrate as. Originally named vibrio subtilis in 1835, this organism was renamed bacillus subtilis in 1872 (microwikicom) one of the first bacteria to be studied one of the first bacteria to be studied this bacteria is a prime example for cellular development. The gram positive unknown was identified to be bacillus subtilis the instructor verified this to be correct the instructor verified this to be correct this deduction was reached with several bits of evidence.
There are numerous reasons for identifying unknown bacteria some of these organisms have distinct qualities that set them apart from one another, such as the exposure to certain environments. The next lab course day the gram staining of escherichia coli and bacillus subtilis was carried out after staining these two species were investigated under the microscope and e coli was short gram-negative bacterium while b subtilis was long gram-positive bacterium. The identification of the unknown a as bacillus subtilis was correct unknown b bacterium, gram negative rods, took multiple tests to identify and was a more intense process compared to unknown a after multiple tests, it was clear that the unknown b bacterium was proteus vulgaris. Lab exercise #3a - id of unknown bacteria 3 iv materials (in addition to supplies found in your supply drawer): cultures of mycobacterium smegmatis, e coli and staphylococcus epidermidis and bacillus subtilis.
These tests were both negative, which confirmed that culture #2 was bacillus subtilis escherichia coli , also known as e coli , is a bacterium that is commonly found in the intestines of humans and animals. Bacillus subtilis used to make several different types of antibiotics including, difficidin, oxydifficidin, bacilli, bacillomyin b, and bacitracin these antibiotics help facilitate quicker healing times for such things as burns, scraps, and certain skin infections (swartzburg, 2009.
The nitrate test confirmed the gram stain, leaving the choices for bacteria b to be bacillus cereus, or bacillus subtilis a gelatin test was completed and the results were positive, which backed up the results of the gram stain and the nitrate test. Bacillus subtilis is positive for starch hydrolysis (pictured below on the left) the organism shown on the right is negative for starch hydrolysis the organism shown on the right is negative for starch hydrolysis. For instance, staphylococcus epidermidis and bacillus subtilis stained purple on the other hand the gram negative escherichia coli that turned colorless, clear, with the application of ethyl alcohol of in addition the colorless gram negative retained the color of the counterstain safranin (red) when applied. Bacillus subtilis, known also as the hay bacillus or grass bacillus, is a gram-positive, catalase-positive bacterium (2) a member of the genus bacillus , b subtilis is rod-shaped, and has the ability to form a tough, protective endospore, allowing the organism to tolerate extreme environmental conditions (3.